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        G418 . sulfate

        更新時間:2022-01-28

        簡要描述:

        Gentamycin-class aminoglycoside antibiotic. Cytotoxic to prokaryotic and eukaryotic cells. Induces apoptosis. Widely employed in the selection of eukaryotic expression vectors, in combination with ……

        型號:廠商性質(zhì):代理商瀏覽量:1039
        Gentamycin-class aminoglycoside antibiotic. Cytotoxic to prokaryotic and eukaryotic cells. Induces apoptosis. Widely employed in the selection of eukaryotic expression vectors, in combination with either aminoglycoside phosphotransferase 3’ or APH II.


        Product Details

        Alternative Name:Geneticin

        Formula:C20H40N4O10 . 2H2SO4

        MW:496.6 . 196.1

        Source:Isolated from Micromonospora rhodorangea.

        CAS:108321-42-2

        RTECS:CB9378500

        Identity:Determined by IR.

        Appearance:White to off-white powder.

        Activity:Potency: ≥700 µg/mg

        Solubility:Soluble in water.

        Shipping:Ambient

        Long Term Storage:-20°C

        Use/Stability:Solutions are stable for 3 to 6 months when stored at -20°C.

        Handling:Avoid freeze/thaw cycles.

        Scientific Background:This product is the disulfide salt form of G418 CAS# 49863-47-0.

        Technical Info/Product Notes:The effective killing concentration of this antibiotic will vary as to the cell type, media, growth conditions, and the cells metabolic rate and position in the cell cycle. Effective concentrations have been reported from 100 µg/ml up to 5 mg/ml or greater. When using G418 in a new cell system, a full dose curve is suggested and with each new lot of G418, several points on that curve should be retested, as the potency determined in a standard B. subtilis assay) may not exactly correlate to your system.

        Regulatory Status:RUO - Research Use Only

        ALX-380-013
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        ALX-380-013


        Product Literature References

        Inhibition of gastric H+,K+-ATPase by new dihydropyrazole derivative KYY-008: T. Fujii, et al.; Biochem. Biophys. Res. Commun. 567, 177 (2021), Abstract;
        Overexpression of ABCC1 Confers Drug Resistance to Betulin: X. Chen, et al.; Front. Oncol. 11, 640656 (2021), Application(s): Cell treatment (carcinoma cell lines: HEK293), Abstract; Full Text
        Transcriptional suppression of ribosomal DNA with phase separation: S. Ide, et al.; Sci. Adv. 6, 42 (2020), Abstract; Full Text
        Remodeling the endoplasmic reticulum proteostasis network restores proteostasis of pathogenic GABAA receptors: Y.L. Fu, et al.; PLoS One 13, e0207948 (2018), Abstract;
        Adropin reduces paracellular permeability of rat brain endothelial cells exposed to ischemia-like conditions: C. Yang, et al.; Peptides 81, 29 (2016), Application(s): Cell culture, Abstract;
        Positive regulation of the enzymatic activity of gastric H+,K+-ATPase by sialylation of its β-subunit: T. Fujii, et al.; Biochim. Biophys. Acta 1858, 1228 (2016), Application(s): Cell culture, Abstract;
        SIRT1 controls cell proliferation by regulating contact inhibition: E.H. Cho, et al.; Biochem. Biophys. Res. Commun. 478, 868 (2016), Application(s): Transfection and establishment of stable SIRT1-overexpression cell line, Abstract;
        Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus: I.S. Harahap-Carrillo, et al.; Vaccines (Basel) 3, 503 (2015), Application(s): Cell Culture, Abstract; Full Text
        The inhibitory effects of anacardic acid on hepatitis C virus life cycle: J. Hundt, et al.; PLoS One 10, e0117514 (2015), Application(s): Cell Culture, Abstract; Full Text


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